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. 2014 Oct 23;10(10):e1004638. doi: 10.1371/journal.pgen.1004638

Figure 6. COSA-1 foci are reduced, and γ-irradiation does not rescue bivalent formation in pph-4.1 mutants.

Figure 6

(A) COSA-1 staining suggests reduced COs in the pph-4.1 mutant. Top, meiotic cells at pachytene in WT (left) and pph-4.1 (right). Bottom, quantitation of COSA-1 focus number. p value of chi-square test is shown. Scale bar, 2 µm. (B) Estimation of age-dependent probabilities of COSA-1 sites to successfully mature into COs. Inset graphs show the sum of the squared differences between the DAPI body-inferred and the COSA-1 focus-inferred chiasma numbers as a function of varying Psuccess. The minimum value (used to generate the bar graph adjustments) is indicated with an arrow. (C) A frequency histogram (normalized to 100%) shows DAPI body numbers for pph-4.1 control (blue bars) and irradiated (green bars) nuclei. R, Spearman's rank correlation coefficient. (D) γ-irradiation restored chiasmata on all 6 chromosome pairs in spo-11(me44) gonads (top) but did not produce additional chiasmata in pph-4.1 gonads (bottom). Scale bar, 5 µm.