Skip to main content
. 2014 Oct 23;10(10):e1004652. doi: 10.1371/journal.pgen.1004652

Figure 5. miR-30c down-regulates the expression of MTDH and HMGA2 by directly targeting their 3′UTRs.

Figure 5

(A) Luciferase activity assays of luciferase reporters with wild type or mutant 3′UTRs of MTDH or HMGA2 were performed after transfection into A549/FHIT or A549/FHIT cells with miR-30c knockdown. (B), (C) Western blot analysis for HMGA2 or MTDH in miR-30c-transfected cells, H1299 and A549. miR-20a was another negative control. (D) MTDH or HMGA2 protein levels in anti-miR-30c-transfected cells stably expressing FHIT. (E) RT-qPCR for MTDH in presence of miR-30c. (F) Correlation between expression levels of miR-30c and MTDH in NSCLC samples (n = 30). Pearson's correlation was used. y, the relative expression level of miR-30c, U6, as an internal control. x, the relative expression level of MTDH, GAPDH, as an internal control. Experiments were performed three times and the data are presented as the mean ± s.d. *P<0.01 and **P<0.001 by Student's t-test.