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. 2014 Oct 23;10(10):e1004655. doi: 10.1371/journal.pgen.1004655

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© 2014 Sohn et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) AvrRps4N-PopP2 fusion construct. (B) Pseudomonas fluorescens Pf0-1(T3S)-delivered AvrRps4N:PopP2^149–488 triggers an EDS1-dependent hypersensitive response (HR) in resistant Arabidopsis accessions. Five week-old Arabidopsis leaves were infiltrated with Pf0-1(T3S) strains expressing indicated avirulence proteins. Empty vector (EV) indicates AvrRps4N encoded by pEDV5 (see Figure S1). The photograph was taken at 24 hours post-infection (hpi). The red asterisks indicate the leaves showing HR. (C) Pf0-1(T3S)-delivered AvrRps4N:PopP2^149–488 triggers an RPS4/RRS1-dependent HR in Ws-2 accession. (D) Pseudomonas syringae pv. tomato (Pto) DC3000-delivered AvrRps4N:PopP2^149–488 triggers RPS4/RRS1-dependent immunity in accession Ws-2. Five week-old Arabidopsis leaves were infiltrated with Pto DC3000 strains and samples were taken at 4 dpi to recover bacteria from infected leaves. The results presented are the mean and standard error of the number of bacterial colonies recovered. Means labeled with the same letter are not statistically different at the 5% confidence level based on Tukey's test.