Table 2. Strains and plasmids used in this study.
| Strains or plasmids | Relevant genotype or phenotype | References |
| Escherichia coli | ||
| BW25113 | F−, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ–, rph-1, Δ(rhaD-rhaB)568, hsdR514 | [50] |
| BW25113 Nx | Nxr-derivative of BW25113 | [19] |
| MG1655 Nx | Nxr-derivative of MG1655 | [51] |
| MG1655 Rf | Rfr-derivative of MG1655 | [51] |
| BL21(DE3) | F−, ompT ΔhsdS gal dcm (DE3) | Novagen |
| β2163 | (F−) RP4-2-Tc::Mu ΔdapA::(erm-pir) (Kn Em) | [49] |
| Salmonella enterica | ||
| St038 | serovar Typhimurium; SGI1+ (Ap Cm Tc Su Sp Sm) | F. Malouin |
| Vibrio mimicus | ||
| VM573 | Patient with diarrhea; USA, 1990s; CT+ TCP+ MGIVmi1+ (Ap Sp Su) | [68] |
| Plasmids | ||
| pVCR94 | IncA/C conjugative plasmid; V. cholerae O1 F1939; Rwanda, 1994 | [19] |
| pVCR94ΔX | Sur-derivative of pVCR94 (Su) | [19] |
| pVCR94ΔX2 | Spr-derivative of pVCR94ΔX (Sp Su) | This study |
| pVCR94ΔX3 | Knr-derivative of pVCR94ΔX (Kn Su) | This study |
| pVCR94Δ25 | pVCR94 ΔX2 Δvcrx025 (Sp Su) | This study |
| pVCR94ΔacaC | pVCR94 ΔX2 ΔacaC (Sp Su) | This study |
| pVCR94ΔacaD | pVCR94 ΔX2 ΔacaD (Sp Su) | This study |
| pVCR94ΔacaCD | pVCR94 ΔX2 ΔacaCD (Sp Su) | This study |
| pVCR94Δacr1 | pVCR94 ΔX2 Δacr1 (Sp Su) | This study |
| pVCR94Δacr2 | pVCR94 ΔX2 Δacr2 (Sp Su) | This study |
| pBAD30 | ori p15A araC P BAD (Ap) | [45] |
| pacaC | pBAD30::acaC (Ap) | This study |
| pacaD | pBAD30::acaD (Ap) | This study |
| pacaCD | pBAD30::acaCD (Ap) | This study |
| pacr1 | pBAD30::acr1 (Ap) | This study |
| pacr2 | pBAD30::acr2 (Ap) | This study |
| pGG2B | pBAD30::setDC (Ap) | [69] |
| pSW23T | oriT RP4; oriV R6Kγ (Cm) | [49] |
| pAH56 | oriV R6Kγ; attP λ; lacI; P tac (Kn) | [70] |
| pJL148 | SPA-tag (CBP-TEV site-3×FLAG) (Kn) | [47] |
| pacaDC 3×FLAG | pAH56::acaDC 3×FLAG (Kn) | This study |
| psetDC 3×FLAG | pAH56::setDC 3×FLAG (Kn) | This study |
| pOPlacZ | pAH56 lacZ (Kn) | This study |
| pPromtraL | pOPlacZ PtraL-lacZ (Kn) | This study |
| pPromtraI | pOPlacZ PtraI-lacZ (Kn) | This study |
| pPromtraF | pOPlacZ PtraF-lacZ (Kn) | This study |
| pPromacr1 | pOPlacZ Pacr1-lacZ (Kn) | This study |
| pProm152 | pOPlacZ P152-lacZ (Kn) | This study |
| pINT-ts | oriR101; cI857; λp R-int λ (Ap Ts) | [70] |
| pET-24b(+) | oriV pBR322; lacI; P T7; 6×His; expression vector (Kn) | Novagen |
| pacaDC 6×His | pET-24b(+)::acaDC 6×His (Kn) | This study |
| pSIM6 | λRed recombination thermo-inducible encoding plasmid (Ts Ap) | [52] |
| pKD3 | PCR template for one-step chromosomal gene inactivation (Cm) | [50] |
| pKD13 | PCR template for one-step chromosomal gene inactivation (Kn) | [50] |
| pVI36 | PCR template for one-step chromosomal gene inactivation (Sp) | [51] |
| pCP20 | Flp recombinase thermo-inducible encoding plasmid (Ts Ap Cm) | [53] |
Ap, ampicillin; Cm, chloramphenicol; Em, erythromycin; Kn, kanamycin; Nx, nalidixic acid; Sp, spectinomycine; Rf, rifampicin; Sm, streptomycine; Su, sulfamethoxazole; Tc, tetracycline; Tm, trimethoprim; Ts, thermosensitive; CT, cholera toxin; TCP, toxin co-regulated pilus.