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. 2014 Oct 23;10(10):e1004717. doi: 10.1371/journal.pgen.1004717

Figure 5. Expression of Abd-B is required in HNCs for the identity of the adult abdominal epithelia.

Figure 5

A) Abd-B expresses in both cell type HNCs and LECs in wild type (top panel). Knockdown of Abd-B using esg-Gal4 show HNCs specific loss of Abd-B protein (middle panel) while in the case of Eip-71CD-Gal4 we see LECs specific loss of Abd-B protein (lower panel). B) It shows cuticle preparations of the adult flies of the corresponding genotype in section A. HNC specific knockdown of Abd-B using esg-Gal4 shows homeotic transformation of A5, A6 and A7 into anterior segments. The A5 segment shows loss of pigmentation (white arrow), A6 segment shows sternite bristles and we see an extra A7 with sternite bristles (red arrow). Bracket sign marks the upper and lower limits of each segment. Knockdown of Abd-B in LECs using Eip-71CD-Gal4 does not show any phenotype (lower most panel). All the cuticles in this figure and subsequent figures are placed anterior at top and dorsal at right.