Figure 4. Intracellular cytokine flow cytometry analysis of the BALF. IL-17⁺ CD4⁺ and CD8⁺ T cells were quantified by multiplying the frequency of each individual cell population by the total cell counts.

Mice were sacrificed at the indicated time points after the 8th exposure. Data are presented as the average ± standard error of measure. n = 7–10 mice/group per time point. ****P≤0.0001, **P≤0.01.