Figure 4. Characterization of the ltgX-atlA region.

A) Fluorometric detection of secreted DNA of mutants created in the ltgX-atlA region was performed as in Figure 2. # indicates a strain which contains a markerless in-frame deletion of the respective gene. * indicates a strain in which the respective gene is disrupted by insertion of an ermC containing plasmid via insertion duplication mutagenesis. $ indicates a strain in which the gene is disrupted by replacement with the ermC cassette via double homologous recombination. yag^#::yag⁺ indicates the complementation mutant of the in-frame deletion of yag by expressing yag from the complementation site between the aspC and lctP genes. ych^& indicates the strain in which ych is disrupted by a transposon insertion. § indicates a Student's T-test P-value≤0.05 compared to wild-type; ‡, indicates no statistical difference compared to WT.