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. 2014 Oct 23;9(10):e109672. doi: 10.1371/journal.pone.0109672

Figure 2. In vitro Activity of LQB-118 on L. braziliensis-infected macrophages.

Figure 2

Monolayer of hamster peritoneal macrophage were infected with L. braziliensis (ratio of 5 parasites/macrophages) and subjected to treatment with the indicated concentrations of LQB-118 for 48 hours at 37°C/5% CO2. A) Macrophage monolayer was stained and the infection index was determined by counting at least 100 macrophage. Nitric oxide production in the supernatants was measured by assaying for nitrite using Griess method (inset) or B) After 48 h of the treatment the monolayers of infected macrophages were washed twice and incubated with Schneider's medium and 20% fetal bovine serum at 28°C for more 48 h and promastigotes were counted (mean ± SD, n = 3). * p<0, 01, ** p<0, 001(LQB-118 treatment in relation to control).