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. 2014 Oct 23;9(10):e110279. doi: 10.1371/journal.pone.0110279

Table 3. Plasmids used in this study.

Plasmid Description Size Reference or source
pEBG1 WT qnrS1-containing plasmid derived from E. coli strain 09/22a, confers resistance to ciprofloxacin, tetracycline and trimethoprim 43,530bp This study
pETS4 pEBG1 plasmid with excised region containing qnrS1 41,387 This study
pINK2002 qnrS1 cloned into the Tet gene of pACYC184 5,038 bp This study
pETStop3 Expression vector (pBAD/Thio-TOPO vector) containing a topB insert 6,773 bp This study
pBAD/Thio-TOPO Arabinose inducible expression vector 4,454 bp Invitrogen
p1ECUMN IncP plasmid derived from E. coli strain UMN026 122,302 bp [36]
p2ECUMN IncX1 plasmid derived from E. coli strain UMN026 33,809 bp [36]
pMG306 Naturally occurring qnrS1-bearing plasmid from a Salmonella isolate [32]
pMB2 Naturally occurring aac(6′)-Ib-cr-bearing plasmid 125 Kb This study
pMB80-2 Naturally occurring conjugative plasmid from enteropathogenic E. coli strain >100 Kb [34]
pGEM-T AmpR; TA-cloning vector 3,000 bp Promega
pACYC184 Tet and Cm resistant cloning vector 4,244 bp NEB