Figure 5. Fyn increases the interaction between ApoER2 and Dab1.

COS7 cells were co-transfected with double-tagged ApoER2 and either Fyn, Dab1, or Fyn and Dab1 and lysates collected in IP buffer after 20 hours. A. Lysates were immunoprecipitated (IP) with either GFP (for Dab1) or rabbit IgG, run on a Western blot, and probed for HA (for ApoER2). Input refers to the starting material that was not subjected to IP. Below are Western blots showing total levels of ApoER2, Dab1, Fyn, and Tubulin. B. The lysates used in A were precipitated with either HA (for ApoER2) or mouse IgG, and analyzed for Dab1-GFP. C. Quantification of lanes 2 and 3 from A. *p<0.05 (t-test). Data are mean +/− SD. N = 3. D. COS7 cells were co-transfected as in A and treated with either DMSO (control) or PP2 for 6 hours. Lysates were collected and immunoprecipitated as in A.