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. 2014 Oct 23;9(10):e110882. doi: 10.1371/journal.pone.0110882

Figure 4. Chromosome association phenotypes of Brd4 and E2 proteins with amino acid substitutions in the hinge.

Figure 4

A, B) Phosphorylation at residue 243 is critical for the co-localization of HPV-16 E2 and Brd4 and association with mitotic chromosomes. COS-7 cells transfected with plasmids expressing HPV-16 wild type E2, S207-mutated E2 (S207A) or S243-mutated E2 (S243A, S243D, S243N, S243E, S243Q) were visualized by confocal microscopy to determine the location of E2 (green), Brd4 (red) and cellular DNA (blue) during mitosis. A) metaphase and B) anaphase.