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. 2014 Oct 23;9(10):e110882. doi: 10.1371/journal.pone.0110882

Figure 5. HPV-16 E2 binds to the chromosomes in a Brd4-dependent manner.

Figure 5

A) C33A cells were transfected with the expression plasmids for EGFP-fused HPV-16 wild type, S243A E2, or EGFP proteins. After fixation, the cells were subjected to fluorescence microscopy after counterstaining of the DNA with DAPI. The cells in metaphase (left) and anaphase (right) are shown. B) Inhibition of Brd4 chromatin binding causes E2 to dissociate from the chromosomes during mitosis. COS-7 cells were transfected with EGFP-tagged E2 and treated with DMSO or 0.5 µM JQ1(+). Location of protein complexes was detected by confocal microscopy for E2 (green), Brd4 (red) and DNA (DAPI).