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. 2014 Oct 23;9(10):e111410. doi: 10.1371/journal.pone.0111410

Figure 1. HBV up-regulated miR499a by increasing its promoter activity.

Figure 1

(A) The relative expressions of miR499a in HepG2.2.15 and HepG2 cell lines were detected by qRT-PCR. (B) The expressions of miR499a in HepG2 cells infected with HBV adenovirus or GFP adenovirus were examined by qRT-PCR. (C) The expressions of miR499a in SMMC-7721 cells transfected with four major HBV protein vector were measured by qRT-PCR. And the results of RT-PCR showed that HBs, HBx, HBc and HBp could be expressed. (D) Luciferase reporter gene assay showed HBV, HBs and HBx could increase the activity of the miR499a promoter. miRNA abundance was normalized to U6 RNA. Empty pCMV-sport6 plasmid was used as a negative control. Statistically significant differences, arbitrarily set to 1.0, are indicated: *p<0.05, **p<0.01, Student's t test.