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. 2014 Oct 23;9(10):e111410. doi: 10.1371/journal.pone.0111410

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© 2014 Xiang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Wound healing assay was performed on SMMC-7721 cells transfected with pTarget or pTarget-miR499a (1×10⁶ per well, 6-well plates) with serum starvation. Red arrows noted the wound edge. (B) Representative images of transwell migration of SMMC-7721 cells transfected by pTarget-miR499a (left, magnification ×200). The mean number of migrated cells in three randomly selected fields were counted under the microscope were shown in bar graph (right). (C) The effects of down-regulated miR499a by transfected miR499a inhibitor were represented by transwell assay.