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. 2014 Oct 23;9(10):e111410. doi: 10.1371/journal.pone.0111410

Figure 4. MAPK6 was the target gene of miR499a.

Figure 4

(A) pGL3-control-MAPK6 plasmid contained a part of 3′UTR of MAPK6 sequence containing putative miR499a binding site. And pGL3-control-MAPK6-mut was mutanted the putative miR499a binding site. Luciferase reporter assays in SMMC-7721 cells, with cotransfection of pGL3-control-MAPK6 or pGL3-control-MAPK6-mut and miR499a as indicated. (B) qRT-PCR results showed the expression level of miR499a in SMMC-7721 cells transfected with pTarget or pTarger-miR499a. (C and D) qRT-PCR and Western blot were performed to analyze mRNA and protein levels of MAPK6 in SMMC-7721 cells transfected with pTarget or pTarger-miR499a. (E) The expression level of miR499a in SMMC-7721 cells transfected with miR499a inhibitor was detected by qRT-PCR. (F and G) The mRNA and protein levels of MAPK6 were indicated by qRT-PCR and Western blot in SMMC-7721 cells after transfection of miR499a inhibitor.