Figure 2. C14dm ⁻ mutants are viable but show defects in growth rate, cell shape and differentiation.

(A) L. major promastigotes were inoculated at 1.0×10⁵ cells/ml and culture densities were measured every 24 hours. After entering stationary phase (72 hours post inoculation, marked by the arrow in A), percentages of dead cells (B), metacyclics (C) and round cells (D) were determined daily. Error bars represent standard deviations from 3 experiments (*: p<0.05, **: p<0.01). Black circle: WT; white circle: c14dm ⁻; black triangle: c14dm ⁻ /+C14DM.