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. 2014 Oct 23;10(10):e1004427. doi: 10.1371/journal.ppat.1004427

Figure 4. Accumulation of 14-methylated sterol intermediates and depletion of ergostane-based sterols in c14dm − mutants.

Figure 4

(A–D) Partial GC chromatograms of lipids from WT (A), c14dm − (B), WT + 0.2 µM ITZ (C), and c14dm − /+C14DM (D) promastigotes. Cholesta-3,5-diene (retention time = 11.82–11.83 minutes) was added to Leishmania samples prior to lipid extraction as an internal standard (arrowheads). Complete chromatograms were provided in Fig. S5–8. These analyses were repeated 3 times and percentages of major sterol species were quantified. Results from one representative experiment were summarized in E. The Roman numerals (VIII–XIII) represent sterol species (Fig. S1) and their corresponding peaks are indicated by arrows in A–D.