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. 2014 Oct 23;10(10):e1004427. doi: 10.1371/journal.ppat.1004427

Figure 6. Altered expression of LPG and GP63 in c14dm − mutants.

Figure 6

(A–B) Whole cell lysates or culture supernatants (LPG in supe.) from log phase (A) or day 3 stationary phase (B) promastigotes (lane 1: WT, lane 2: c14dm −, lane 3: c14dm − /+C14DM) were analyzed by Western blot, using antibodies against LPG, GP63, or α-tubulin. (C–D) The relative abundance of LPG (C) and GP63 (D) was normalized in log phase, stationary phase, and metacyclic parasites (black bars: WT; white bars: c14dm −; grey bars: c14dm − /+C14DM). Error bars represent standard deviations from 3 experiments (*: p<0.05, **: p<0.01).