Table 1. Susceptibility of c14dm ⁻ mutants to ITZ and Amp B.

Drug	IC25 (µM ± SD)			IC50 (µM ± SD)			IC90 (µM ± SD)
	WT	c14dm −	Ratio	WT	c14dm −	Ratio	WT	c14dm −	Ratio
ITZ	0.12±0.10	0.60±0.20	1/5	0.40±0.10	2.0±0.3	1/5	10±1.4	10.0±1.0	1
Amp B	0.01±0.006	0.1±0.1	1/10	0.025±0.01	0.9±0.3	1/36	0.05±0.015	5.0±0.4	1/100

L. major WT or c14dm ⁻ promastigotes were inoculated in M199 medium at 2.0×10⁵ cells/ml in various concentrations of ITZ or Amp B. Culture densities were examined after 48 hours and IC values to achieve 25%, 50%, and 90% inhibition were determined by comparing to control cells grown in the absence of inhibitors. SD: standard deviations from 3 independent experiments. Ratios of IC (WT)/IC (c14dm ⁻) were indicated.