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. 2014 Oct 23;10(10):e1004480. doi: 10.1371/journal.ppat.1004480

Figure 5. Ethanol acts through the Wsp system.

Figure 5

A. Attachment of P. aeruginosa strain PAO1 WT, ΔwspR and ΔwspA mutants to the bottom of a polystyrene dish during growth in M63 medium with glucose and casamino acids, and with vehicle (Control) or with 1% ethanol for 6 hours. B. The number of cells per field for each condition was enumerated. Images and data are representative of results from more than three separate experiments. Significance determination was based on an ordinary one-way ANOVA followed by Sidak's multiple comparisons test for each intrastrain comparison; ***, P<0.001. C. ΔwspR and ΔwspFR strains expressing WspR-E253A-YFP were grown without and with 1% EtOH, and the number of cells with fluorescent clusters were counted out of a total of approximately 100 cells examined per condition across two experiments.