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. 2014 Oct 23;10(10):e1004466. doi: 10.1371/journal.ppat.1004466

Figure 3. E7 epidermis produce CCL2 and CCL5 and MCs express the corresponding receptors.

Figure 3

(A) C57 and E7 whole skin, or (B) C57 and E7 epidermis, were analyzed for SCF, CCL5 (Rantes) and CCL2 (MCP-1) gene expression. Data are combined from 2 independent experiments. CCR1 and CCR5 (two Rantes receptors) and CCR2 (MCP-1 receptor) gene expression in (C) BMCMCs after 72 h culture in E7 or C57 ear skin culture supernatant or medium (n = 10 different batches of BMCMCs; data are combined from 4 independent experiments. ND, not detectable) and (D) Mast cells sorted from E7 ear skin (n = 6 independent experiments, each with 4 or 5 mice pooled per treatment). Gene expression was performed by real time-PCR relative to the RL32 housekeeping gene. *p<0.05; **p<0.01; ***p<0.001; or ****p<0.0001 by unpaired t-test for indicated comparisons; ns  =  not significant.