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. 2014 Jun 20;155(9):3227–3237. doi: 10.1210/en.2014-1016

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Copyright © 2014 by the Endocrine Society

This article has been published under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright for this article is retained by the author(s). Author(s) grant(s) the Endocrine Society the exclusive right to publish the article and identify itself as the original publisher.

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Figure 1. — Components of the vitamin D-endocrine system in C2C12 muscle cells. A, C2C12 cells express CYP27B1, VDR, and CYP24A1 mRNA as seen on semiquantitative PCR. Duplicates for each have been shown. B, Expression of VDR mRNA is stimulated in a dose-dependent fashion by 48 hours of treatment with 1,25(OH)₂D (data are mean ± SEM, n = 3 per group). C, VDR mRNA drops sequentially during C2C12 differentiation from myoblasts to myotubes (P < .005). Western blotting (D) and densitometric quantitation (E) show that VDR expression (normalized for β-actin) increased 2.2-fold in response to 72 hours of treatment with 1,25(OH)₂D in C2C12 myotubes (P < .005, n = 3 per group).