Table 6.

Clinical chemistry, hematology and immunology

Assay	Aging phenotype	Intervention	Strain, sex	Treatment effects in aged mice	Treatment effects in young mice	Reference
Blood cell counts	Reduced red blood cell couns, increased white blood cell counts and platelets	Aging cohorts: oral rapamycin (14 ppm) for approx. 1 year starting at 4 months, 13 months or 25 months; young animals: oral rapamycin (14 ppm) for 3 months starting at 12 weeks of age	Male C57BL/6J Rj	Rapamycin increased red blood cell counts; no effect on white blood cell counts and platelets	Increase of red blood cell counts	[13]
Clinical chemistry	Increased plasma sodium, calcium, chloride, total protein, albumin, alkaline phosphatase, and α-amylase; decreased triglycerides	Oral rapamycin (14 ppm) for approx. 1 year starting at 4, 13 or 25 months	Male C57BL/6J Rj	No amelioration of these aging traits	Not examined	[13]
Immunology (FACS)	Increased CD25+CD4+ T cell population; decreased γδ T cell population; increased CD44hi T cell populations	Aging cohorts: oral rapamycin (14 ppm) for approx. 1 year starting at 4, 13 or 25 months; young animals: oral rapamycin (14 ppm) for 3 months starting at 12 weeks of age	Male C57BL/6J Rj	Decreased CD25+ CD4+ T cell population; increased γδ T cell population; decreased CD44hi T cell populations	Decreased CD25+CD4+ T cell population; no effect on γδ T cell and CD44hi T cell populations (but see [48] and [56])	[13]
	Decreased CD4+ T lymphocytes; decreased NK cells and NK/CD11b+ cells; increased IgDhi B cells and MHCIIhi B cells			No measurable amelioration of aging traits	Not examined
Immunoglobulin measurements	Increased plasma immunoglobulin concentrations			Tended to decrease plasma immunoglobulins (in part)	Not examined