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. Author manuscript; available in PMC: 2015 Jan 31.
Published in final edited form as: Nat Commun. 2014 Jul 31;5:4511. doi: 10.1038/ncomms5511

Figure 2. SOX2 is required for cutaneous SCC initiation and growth.

Figure 2

(a) Tumor growth curves of human SCCs infected with lentivirus expressing short hairpin RNA (shRNA) against SOX2 along with nuclear red fluorescent protein (H2B-RFP; shSOX2) or scrambled control shRNA along with nuclear green fluorescent protein (H2B-GFP; shSCR) followed by transplantation onto Nude recipient mice. Data are represented as mean with error bars indicating ± s.e.m. (n=6, *P<0.05, Student’s t-test). (b) Doxycycline-inducible knockdown of SOX2 in established human SCC xenografts. Data are represented as mean with error bars indicating ± s.e.m. (n=10, *P<0.05, Student’s t-test). (c–e) Quantitative analysis of proliferative Ki67 (c), mitotic phospho-Histone H3(Ser10) pH3 (d), and apoptotic activated Caspase-3 (Casp3) (e) positive cells in shSOX2;H2B-RFP or shSCR;H2B-GFP transduced A431 xenografts. Scatter plots illustrate the percentage of infected parenchymal cells that are positive for the respective marker in n>200 microscopic fields. Horizontal bars indicate mean ± 95% CI. (*P<0.05 Mann-Whitney non-parametric t-test). (f–g) Analysis of clonal growth competition assay at 2 and 4 weeks after intradermal transplantation when 1–2% of A431 cells have been transduced with shSOX2;H2B-RFP and shSCR;H2B-GFP (n=6). (f) Scatter plots illustrate clone size distributions. Horizontal lines represent mean with error bars indicating ± 95% CI. (g) Line graphs showing average clone size as a function of time (± s.e.m). (f–g) P values were obtained by Mann-Whitney non-parametric t-tests.