FIGURE 1.

Inhibition of Hmp-supported NO resistance by CORM-3 in E. coli cultures. Growth of aerobic cultures in defined minimal medium was monitored every hour using a Klett meter. A, cultures of the E. coli wild type (●) or an hmp mutant (♢) upon addition of 100 μm DETA NONOate (dotted arrow) were compared with a wild type culture growing in the absence of DETA NONOate (○). B, wild type cultures supplemented with 100 μm DETA NONOate (as in A) plus 5 μm CORM-3 (solid arrow) (■), were compared with cultures containing 100 μm DETA NONOate (●) or 5 μm CORM-3 (▿) only, added in the same conditions. C, as in B, but 10 μm CORM-3 was added. D, as in B but 10 μm iCORM-3 instead of CORM-3 was added. Bars represent the standard deviation of three independent experiments.