FIGURE 2.

CORM-3 fails to inhibit the NO detoxification activity of purified Hmp in vitro. O₂ consumption (solid line) and NO accumulation (dashed line) were polarographically recorded simultaneously. Reaction mixes containing 1 μm Hmp and 500 μm NADH final concentrations in 50 mm Tris-HCl, 50 mm NaCl (pH 8.0) at 37 °C were treated with repeated additions of 25 μm PROLI-NONOate (solid arrows) in the absence (A) or presence of 200 μm CORM-3 (dotted arrow) (B), or CO-saturated buffer was added to a final concentration of 100 μm (C) or 1 mm (D). O₂ concentration consumed after additions of PROLI-NONOate is shown in A. The experiments were repeated at least three times with similar results.