FIGURE 5.

Formation of CO-reduced Hmp upon addition of CORM-3 in E. coli cellular extracts. Hmp (4.7 μm) was added to E. coli hmp soluble extracts containing 500 μm NADH. A, Hmp absolute spectra were recorded after 10 min incubation at room temperature to promote reduction (dashed line), then immediately after addition of 100 μm CORM-3 (dotted line) and every min for 20 min and every 5 min for an additional 20 min (solid lines). Inset shows the amplification of the α,β region. B, difference spectra after addition of CORM-3. Insets show amplification of the α,β region (above), and the CO release time course from CORM-3 (below). Curved arrows indicate the direction of the changes.