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E. coli membrane suspensions fail to promote CO releasing from CORM-3. Hmp (4.7 μm) was added to E. coli membrane suspension containing 1 mm NADH. Hmp absolute spectra were recorded after 10 min incubation at room temperature to promote reduction (dashed line), then immediately after addition of 100 μm CORM-3 (dotted line), and after 40 min (solid line). Inset shows the amplification of the α,β region.
