FIGURE 2.

smARF expression leads to translocation of Parkin from the cytosol to mitochondria. A, HeLa cells transduced with eGFP-FLAG-Parkin or eGFP lentivirus for 24 h were transfected with PINK1-Myc, pcDNA, p19WT, p19M1I, p19M45I, p19ΔNT, or PINK1-c-Myc and processed for immunofluorescence after 24 h using cytochrome c (CytC) and p19 or p14 antibodies. Micrographs of both eGFP and eGFP-FLAG-Parkin are shown for p19WT, p19M1I, p19M45I, and p19ΔNT. Only the eGFP-Parkin micrograph is shown for the pcDNA condition. Scale bar, 20 μm for low power and 5 μm for zoom. B, histogram showing the proportion of transfected cells with eGFP-FLAG-Parkin co-localizing with cytochrome c. For each construct, at least 100 cells were counted per condition per experiment. All columns are compared with pcDNA. n = 3. **, p < 0.01; ***, p < 0.001. Error bars, S.E.