TABLE 3.
Transverse relaxation rate constants for AlkB-ΔN11/ligand complexes
The zero- and double-quantum relaxation rate constants (RZQ and RDQ, respectively) were measured using a Hahn-echo experiment (53). As in Table 2, saturating concentrations of each titrant were added in succession to the previous complex.
| Ligand | Met-49 | Met-57 | Met-61 | Met-92 | |
|---|---|---|---|---|---|
| RZQ (s−1) | Zn(II) | 27.0 ± 1.1 | 27.0 ± 1.1 | 94.0 ± 0.2 | 32.2 ± 2.0 |
| 2OG | 54.6 ± 3.6 | 39.1 ± 4.6 | 59.4 ± 7.2 | 27.6 ± 4.0 | |
| 5′-CAmAAT-3′ | 30.5 ± 1.0 | 45.6 ± 0.9 | 33.8 ± 1.1 | 28.8 ± 1.1 | |
| RDQ (s−1) | Zn(II) | 80.4 ± 0.7 | 80.4 ± 0.7 | 97.9 ± 0.9 | 56.9 ± 1.1 |
| 2OG | 322 ± 14 | 83.1 ± 7.7 | 80.8 ± 13.1 | 41.7 ± 4.9 | |
| 5′-CAmAAT-3′ | 38.6 ± 0.3 | 67.2 ± 1.8 | 47.6 ± 2.6 | 65.3 ± 2.1 |