FIGURE 2.
MTHFD1 protein levels in liver nuclei from wild type and Mthfd1gt/+ knockdown mice after 22 ± 2 weeks on diet. A, nuclear tissue lysate was probed with rabbit anti-human Lamin A/C antibody as the loading control. The same membrane was probed with mouse anti-human glyceraldehyde-3-phosphate as a control for nuclei purity. B, immunoblot analysis of total liver extract from wild type and Mthfd1gt mice. Total cell lysate was probed with mouse anti-human glyceraldehyde 3-phosphate as the loading control. Both membranes were treated with polyclonal sheep anti-mouse MTHFD1 antibody to detect MTHFD1 protein.