TABLE 1.

Nuclear liver folate levels are resistant to folate depletion

Male mice were weaned onto and maintained on folate-sufficient and folate-deficient diets for 22 ± 2 weeks. Total cellular and nuclear folates n = 3–8 per group were measured. Values represent the mean ± S.E. NS, no significant difference.

Genotype	AIN-93G (control)		AIN-93G − folic acid (FD)
	Whole cell folate	Nuclear folate	Whole cell folate	Nuclear folate
	fmol/μg protein	fmol/μg protein	fmol/μg protein	fmol/μg protein
Mthfd1+/+	99.0 ± 14.4	29.2 ± 6.2	63.4 ± 6.2	28.9 ± 5.2
Mthfd1gt/+	104.3 ± 2.7	32.3 ± 5.2	44.7 ± 9.5	28.8 ± 8.0
p Value, diet effect			<0.001	NS
p Value, genotype effect			NS	NS
p Value, diet x genotype effect			NS	NS