TABLE 2.

Nuclear folate levels are not affected by cell cycle arrest or folate depletion in MCF-7 cells

Values are represented as the mean ± S.D. with n = 3. Intracompartmental differences with cell cycle and folate content of the media were analyzed using Student's t test and a Bonferroni correction for multiple (9) comparisons, and data were considered significantly different if p < 0.05. p values are shown for significant comparisons (6R,S)-5-CHOTHF, (6R,S)5-formyltetrahydrofolate.

Culture media	Cell cycle arrest	Whole cell folate	Nuclear folate
		fmol/μg protein	fmol/μg protein
α-MEM	G1	24.6 ± 0.9	1.40 ± 0.19
	S	23.8 ± 1.2	1.67 ± 0.29
Folate-replete	G2/M	23.3 ± 0.9	1.65 ± 0.25
Defined MEM with 5 nm (6R,S)-5-CHOTHF	G1	13.0 ± 0.7 (p < 0.001 compared to replete)	0.76 ± 0.06 (p < 0.05 compared to replete)
	S	12.9 ± 0.4 (p < 0.001 compared to replete)	1.12 ± 0.17
Folate-depleted	G2/M	12.5 ± 0.8 (p < 0.001 compared to replete)	0.84 ± 0.04 (p < 0.05 compared to replete)