FIGURE 4.

MALDI mass spectrometric data of N-linked glycans from recombinant TFF2 wild type and truncation probes. A, full-length TFF2 probe (TFF2-Fl). B, N-terminal P1 domain probe (N-P1). C, C-terminal P2 domain probe with designed N-glycosylation site (D87N/R89T): C-P2*. The inserted glycan structure refers to signals marked by an asterisk. Molecular ions referring to LacdiNAc-positive glycans are written in boldface. The signals correspond to the sodium adducts of molecular ions (M+Na) of permethylated glycans. a.u., arbitrary units.