TABLE 1.
N-Linked glycans on recombinant human TFF2 constructs expressed in HEK-293 cells
Glycans with masses in boldface were demonstrated by MS/MS to express terminal LacdiNAc, fucosylated LacdiNAc, or sialinated LacdiNAc. M+Na, M+Na-32, and M+Na-54 molecular ion species shown in Figs. 4 and 6 are listed as M+Na. Semiquantitative data from MALDI-MS of permethylated glycans are presented as ++ (major, base peak), + (>5% of base-peak intensity), (+), (minor species with <5% of base-peak intensity).
| Exp. rel. mass M+Na (m/z) | Glycan |
hTFF2 constructs |
|||||
|---|---|---|---|---|---|---|---|
| Fl | N-P1 | C-P2*(D87N) | N-P1 (W68Q/F59Q) | FL-(C52G) | Fl-(C42G)a | ||
| 1526.1 | H5N2 | − | − | − | ++ | − | − |
| 1730.2 | H6N2 | − | − | − | ++ | − | − |
| 1934.3 | H7N2 | − | − | − | + | − | − |
| 2138.5 | H8N2 | − | − | − | + | − | − |
| 2342.6 | H9N2 | − | − | − | + | − | − |
| 2214.3 | F2H4N4 | + | − | + | − | − | − |
| 2430.8 | S1H5N4 | − | − | − | − | + | − |
| 2459.4 | F2H4N5 | − | − | + | − | + | − |
| 2500.4 | F2H3N6 | + | + | + | − | + | − |
| 2633.5 | F3H4N5 | + | (+) | + | − | (+) | − |
| 2674.5 | F3H3N6 | ++ | ++ | ++ | − | + | − |
| 2791.9 | S2H5N4 | − | − | − | (+) | ++ | + |
| 2820.6 | S1F2H4N5 | + | + | + | − | − | − |
| 2861.6 | S1F2H3N6 | + | + | + | − | − | − |
| 2994.6 | S1F3H4N5 | (+) | (+) | − | − | − | − |
| 3164.8 | F3H3N8 | + | + | (+) | − | − | − |
| 3242.0 | S2H6N5 | − | − | − | (+) | + | − |
| 3310.8 | S1F2H4N7 | (+) | + | + | − | − | − |
| 3417.1 | S2F1H6N5 | − | − | − | − | (+) | − |
| 3484.9 | S1F3H4N7 | (+) | (+) | + | − | − | − |
| 3602.1 | S3H6N5 | − | − | − | (+) | ++ | + |
| 3866.2 | S2F1H7N6 | − | − | − | − | (+) | − |
| 3963.2 | S4H6N5 | − | − | − | − | + | (+) |
a Only two major glycan species were detectable as sample amounts of Fl-(C42G) were significantly lower than those of other TFF2 probes.