TABLE 2.
WFA affinity chromatography of HEK-293 secretome
The list summarizes those proteins identified in fractions eluted with 0.1 m lactose and 0.1 m GalNAc. Proteins designated in boldface were found in both technical replicates. GO data refer to proteins forming ECM components or being functionally ECM-related (ECM), to secreted proteins (sec), and to glycosylated proteins (N, O).
| Designation | ECM component or ECM-related | Secreted protein | Glycosylated (N- or O-linked) |
|---|---|---|---|
| LG3BP (galectin-3-binding protein) | ECM | sec | N |
| PXDN (peroxidasin) | ECM | sec | N |
| C1R (C1 component) | serine protease | sec | N |
| SMOC1 (SPARC-related modular calcium binding protein 1) | ECM | sec | N |
| VTNC (vitronectin) | ECM | sec | N,O |
| NID1(nidogen-1) | ECM | sec | N, O |
| NID2 (nidogen-2) | ECM | sec | N, O |
| LAMA1 (laminin-α1) | ECM | sec | N |
| LAMA4 (laminin-β4) | ECM | sec | N |
| LAMAB1 (laminin-β1) | ECM | sec | N |
| LAMAC1 (laminin-γ1) | ECM | sec | N |
| TIMP1 (tissue metalloproteinase 1) | ECM | sec | N |
| NPTX1(neuronal pentraxin 1) | synaptic regeneration | sec | N |
| FBN2 (fibrillin-2) | ECM | sec | N |
| FBLN1 (fibulin-1) | ECM | sec | N |
| CLUS (clusterin) | extracell. chaperone | sec | N |
| LTBP4 (latent transforming growth factor β−binding protein 4 | ECM | sec | N |
| AGRIN | ECM | sec | N |
| COCH (cochlin) | ECM | sec | N |
| COL6A1 (collagen 6α1) | ECM | sec | N |
| FINC (fibronectin) | ECM | sec | N, O |
| γ-Glutamyl hydrolase | γ-glutamyl peptidase | sec | N |
| Sulfhydryl oxidase 1 | protein disulfide isomerase | sec | N |