TABLE 2.
Primers used in this study
| Primer | Sequence (5′–3′) | |
|---|---|---|
| Northern probe primer | ||
| hla-F | Forward | AGAAAATGGCATGCACAAAAA |
| hla-R | Reverse | TGTAGCGAAGTCTGGTGAAAA |
| RNAIII-F | Forward | GTGATGGAAAATAGTTGATGAG |
| RNAIII-R | Reverse | GTGAATTTGTTCACTGTGTCG |
| Real-time RT PCR primer | ||
| capA | Forward | GAAAATACGCAACTTATCAACATCCA |
| capA | Reverse | TTTTTTCCGAATCTTGTTTATGACC |
| crtM | Forward | ACGCTTTTGACTTGTTACCAGAAGA |
| crtM | Reverse | AAATTGCCCAAACCGCTTT |
| Hla | Forward | GAACCCGGTATATGGCAATCAA |
| Hla | Reverse | GGAAGTTCTCTGCTGCTTTCATAG |
| RNAIII | Forward | AGGAGTGATTTCAATGGCACAAG |
| RNAIII | Reverse | TGTGTCGATAATCCATTTTACTAAGTCA |
| Psma | Forward | ATCAACAACTCATCACTATGTTAAATCAAC |
| Psma | Reverse | GCCATCGTTTTGTCCTCCTGT |
| gyrB | Forward | GACTGATGCCGATGTGGA |
| gyrB | Reverse | AACGGTGGCTGTGCAATA |
| EMSA primer | ||
| agr P2/3 | Forward | CCATCACATCTCTGTGATCTAG |
| agr P2/3 | Reverse | CTCTCCTCACTGTCATTATACG |
| capA p | Forward | ATCATATGATTATAAGCAATAA |
| capA p | Reverse | GTAATACTTCTTTAATTTTTG |
| hla p | Forward | TAATTAATACCCTTTTTCTCTATTTC |
| hla p | Reverse | GTTACTGAGCTGACTATACGTGTTTTCAT |
| psma p | Forward | CAAATTCATGAGCTTAACCTC |
| psma p | Reverse | GAATTGTTCGATTAAGCTTTTG |