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. 2014 Sep 10;289(43):29728–29738. doi: 10.1074/jbc.M114.603084

FIGURE 2.

FIGURE 2.

Jasmonate profile in wounded rosette leaves of CYP94 T-DNA insertion mutants and overexpressing lines. Rosette leaves were wounded twice across the midrib with a hemostat. Damaged leaves were harvested for JA extraction at indicated times after wounding and analyzed by LC-MS/MS. A and B, JA-Ile and 12OH-JA-Ile levels in wounded (2 h) and unwounded (0 h) leaves of WT, cyp94b3-1, cyp94b1-1, and cyp94b1-1cyp94b3-1 plants. *, p < 0.001; Student's t test. C and D, time course of JA-Ile and 12OH-JA-Ile accumulation in wounded leaves of plants overexpressing CYP94B3 (94B3OE) or CYP94B1 (94B1OE). *, p < 0.01; Student's t test. E and F, relative abundance of 12COOH-JA-Ile in wounded leaves of WT, cyp94b1-1cyp94b3-1, 94B3OE, and 94B1OE at various times after wounding. *, p < 0.05 between WT and 94B3OE; Student's t test. Each data point represents the mean ± S.D. of three biological replicates.