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NMR spectroscopy of HMG-D and HMG-D lacking the acidic tail (HMGD Δ12). a, 15N HSQC spectra. b, chemical shift differences (Δδ = [(ΔδH)2 + (0.15 × ΔδN)2]½ (37)); mean Δδ 0.028 ppm (dotted line). Inset ribbon structures are based on 1QRV. Log10(Δδ + 1) was converted to a rainbow color ramp (blue = least shifted, red = most shifted residues); DNA is shown (gray) to demonstrate the similarity between the acidic-tail-binding and DNA-binding surfaces.
