FIGURE 6.

¹⁵N HSQC spectra of single-box HMG proteins in the absence and presence of phosphorylation. a, chemical shift differences (Δδ = [(Δδ^H)² + (0.15 × Δδ^N)²]^½ (37)) between HMG-D and the phosphorylated form, HMG-D-P; Ser¹⁰³ and Ser¹¹¹ are phosphorylated (Fig. 5). b, ¹⁵N HSQC spectra of HMG-D (blue), HMG-D-P (red), and HMG-D Δ12 (black) for comparison of shift trajectories. c, ZmHMGB1 (blue) and the phosphorylated form, ZmHMGB1-P (red); Ser¹⁴⁹ undergoes a significant chemical shift change indicative of phosphorylation, as does another peak that cannot be assigned due to peak overlap in the spectrum of the unphosphorylated protein. d, chemical shift differences as in a.