FIGURE 7.

AFM analysis of a membrane patch prepared from the LH1-limited mutant DD13(pRKΔTΔX). A, three-dimensional representation of a membrane patch exhibiting examples of an intact RC-LH1 complex (magenta arrow), an isolated RC (white arrow), an RC-LH1 complex missing the H-subunit (blue arrow), and an empty LH1 ring (red arrow). B, schematic diagram to illustrate the expected height difference of an isolated RC, which would rest directly upon the mica substrate, in comparison with an intact RC-LH1 complex where the C-terminal region of the LH1 ring rests on the mica, holding the RC above it.