FIGURE 4.

LSD1 regulation of lipogenic gene expression requires its demethylase activity. A, immunoblots for HA-tagged WT or A539E (AE) LSD1 proteins that are resistant to shRNA#2. B and C, effects of the indicated doses of LSD1-WT (B) or -AE (C) on LSD1 knockdown-induced inhibition of the FAS promoter as examined by luciferase reporter assays. D, effects of trans-2-phenylcyclopropylamine (TCP) (0.2 mm) on the activity of the FAS promoter after 18 h of treatment. E and F, effects of LSD1 overexpression by adenovirus in primary rat hepatocytes on indicated gene expression by qRT-PCR (E) and triglyceride levels (F). The data represent means ± S.D. (n = 3). *, p < 0.05; #, p < 0.01; &, p < 0.001 versus NS or GFP; §, p < 0.05 versus vector.