FIGURE 1.

GCase-GFP transfection constructs for Gba1 null/null cells. On the left are the resultant GCases following the various deletions, indicated as GCaseZZZ to show the encoded mature amino acid contents of the expressed enzymes. The constructs are labeled GCase-XX or -XXX to designate the number of amino acids that were deleted from the -COOH end of the mature 497-amino acid sequence of the WT GCase. GFP was cloned in frame with the WT or GCase-XX or -XXX. These constructs were subsequently transfected into Gba1^−/− fibroblasts.