FIGURE 3.

Mature WT GCase amino acid sequence highlighting the regions targeted for mutagenesis, expression, and binding analyses. The amino acid sequence of GCase is marked with various dark red arrows indicating the number of amino acids deleted from the COOH-terminal end of the enzyme for the GFP reporter. Shown in orange is a typical dileucine sequence for indirect targeting of membrane-bound lysosomal proteins. Underlined in red are the amino acids for targeted mutagenesis and binding studies to ldLIMP-2. These sequences have very high conservation across species (data not shown).