FIGURE 5.

Co-localization (Pearson) indices of GCases with specific alanine substitutions in the WT TKD sequence. GCase/mutant constructs were transfected into Gba1^−/− fibroblasts, and co-localization was evaluated with directly labeled anti-mouse calreticulin (rhodamine; red) antibody for ER/Golgi, directly labeled Lamp1 (rhodamine; red) for lysosome (Lyso), and directly labeled anti-human GCase (FITC; green) for GCase. The alanine (A) substitutions are as altered from the WT TKD sequence. For the AAA and AKA GCases, the majority of the intracellular enzymes co-localized to the ER/Golgi (ER), whereas the TAD GCase showed about 50% of the total intracellular enzyme localized to the lysosome (Lyso). Co-localization indices (Pearson) were calculated (n = 5) and presented as the mean ± S.E. (error bars)