TABLE 1.
Synthetic peptide characteristics
Peptides were custom-synthesized by American Peptide Co. The boldface letters indicate the differences from the WT sequences of DSPIIV or DDQRLLL. The final peptide in each group is an unrelated peptide in GCase.
| Sequence | GCase amino acids | Mr (FITC)a | Mr (non-labeled) | Puritya |
|---|---|---|---|---|
| % | ||||
| DSPIIV | 399–404 | 1032.2 | 642.8 | 95.3/98.0 |
| ASPIIV | 399–404 | 998.1 | 578.8 | 95.0/98.1 |
| DSPAIV | 399–404 | 990.1 | 600.7 | 96.5/98.2 |
| DSPIAV | 399–404 | 990.1 | 600.7 | 95.3/97.7 |
| DSPAAV | 399–404 | 948.1 | 514.6 | 95.4/95.1 |
| SKDVPL | 465–470 | 1047.2 | 657.8 | 96.6/98.0 |
| DDQRLLL | 282–288 | 1261.4 | 872 | 95.3/97.1 |
| ADQRLLL | 282–288 | 1226.4 | 828 | 96.1/97.6 |
| DAQRLLL | 282–288 | 1226.4 | 828 | 95.7/97.1 |
| DDQRAAA | 282–288 | 1144.2 | 745.8 | 95.9/98.0 |
| DFIARDL | 258–264 | 1247.4 | 849 | 95.1/97.5 |
a Quality control: HPLC grade, purity: 95.1–98.8%, MS analysis/desalted/lyophilized into powder.