FIGURE 6.

A 160-bp conserved region of the fascin promoter is sufficient to induce luciferase expression in response to IL-6. A, a 160-bp conserved region of the human fascin promoter containing an overlapping STAT site and NFκB site was subcloned into the luciferase reporter tkluc to generate FasP-luc. B, luciferase assays were performed in MDA-MB-231 cells transfected for 24 h with empty vector tkluc (negative control), M67 (positive control), which contains four Stat3 binding sites, and FasP-luc. Cells were treated with IL-6 for 6 h. C, MDA-MB-231 cells were transfected with tkluc or FasP-luc and treated with IL-6, TNF-α, or IL-6 and TNF-α together for 6 h. Results represent the averages and S.D. of at least three independent experiments.