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. 2014 Sep 15;289(43):30133–30143. doi: 10.1074/jbc.M114.570440

FIGURE 2.

FIGURE 2.

CRMP4 regulates axon outgrowth and growth cone size. A, hippocampal neurons from CRMP4+/+ and CRMP4−/− mice were cultured for 3 DIV and stained with anti-βIII-tubulin antibody. Scale bar, 25 μm. B and C, the average axon length (B) and average neurite length (C) were quantified from cultures derived from CRMP4+/+ or CRMP4−/− mice. D, hippocampal neurons stained with anti-βIII-tubulin antibody (green) and rhodamine phalloidin (red) were classified as blunt ended, filopodial, or lamellipodial. Scale bar, 5 μm. E–J, hippocampal neurons from CRMP4+/+ and CRMP4−/− mice (E–G) or wild-type mice infected with GFP or CRMP4 (H–J) were fixed at 3 DIV and co-stained with an anti-βIII-tubulin antibody and rhodamine phalloidin (E and H). Growth cone morphology (F and I) and size (G and J) were quantified. Determinations are mean ± S.E. from three experiments performed on 30 cells per experiment. *, p < 0.05; **, p < 0.01 by Student's t test compared with control. Error bars represent S.E. Scale bar, 5 μm.