FIGURE 8.

CRMP4 activity toward microtubules promotes growth cone expansion and axon outgrowth. A and C, hippocampal neurons from CRMP4−/− mice transduced with GFP or CRMP4 rescue constructs and stained with anti-βIII-tubulin (A) or anti-βIII-tubulin and rhodamine phalloidin (C). Scale bar, 25 (A) and 5 μm (C). B and D, quantitation of axon outgrowth per cell (B) and growth cone area per cell (D) for cultures derived from CRMP4−/− mice and transduced with GFP or CRMP4 rescue constructs. Values are mean ± S.E. and are from three experiments performed on 30 cells per experiment. Statistical analysis was performed by one-way ANOVA with a Tukey's post hoc test compared with GFP control. *, p < 0.05; **, p < 0.01. Error bars represent S.E.