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. 2014 Oct 24;4:6760. doi: 10.1038/srep06760

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(a) Nb-encoding DNA is in vitro transcribed to (b) mRNA, and (c) ligated to a 3′ puromycin (P) containing DNA linker. (d) Following in vitro translation and fusion formation, (e) reverse transcription is performed to synthesize cDNA. (f) mRNA-cDNA-Nb complexes are exposed to the antigen (Ag) of interest, which is immobolized on a solid-phase affinity matrix. Following washes and elution, PCR is performed to retrieve full-length Nb-encoding amplicons (N/C – N- & C-termini of displayed Nb).